@article{4,
  author = {Rachel Kaletsky and Victoria Yao and April Williams and Alexi Runnels and Alicja Tadych and Shiyi Zhou and Olga Troyanskaya and Coleen Murphy},
  title = {Transcriptome analysis of adult Caenorhabditis elegans cells reveals tissue-specific gene and isoform expression.},
  abstract = { <p>The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans{\textquoteright} four major tissues (or "tissue-ome"), identifying ubiquitously expressed and tissue-specific "enriched" genes. These data newly reveal the hypodermis{\textquoteright} metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms. Finally, we developed a machine learning-based prediction tool for 76 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-β activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals.</p>
 },
  year = {2018},
  journal = {PLoS Genet},
  volume = {14},
  pages = {e1007559},
  month = {2018 Aug},
  issn = {1553-7404},
  doi = {10.1371/journal.pgen.1007559},
  language = {eng},
}