Protocols

CeAid (C. elegans Application for inputting data)

CeAid (C. elegans Application for inputting data)

Assays performed on model organisms, including C. elegans, often require a large sample size and daily recording of data. To minimize the amount of time that the experimenter spends logging data, we developed CeAid (C. elegans Application for inputting data) to automate the data entry process [1].

CeSnAP (C. elegans Snapshot Analysis Platform)

CeSnAP (C. elegans Snapshot Analysis Platform)

We have created a ML-based algorithm, CeSnAP, paired with a rapid snapshot acquisition technique that serves as a simple and versatile workflow to quantify C. elegans behavioral phenotypes [1]. CeSnAP can be used for smart analysis of videos or snapshots upon training of a convolutional neural network (C-NN).

Learning & Memory

Learning & Memory

Here we describe methods to test C. elegans associative learning and short- and long-term associative memory. These population assays employ the worm's ability to chemotax toward volatile odorants, and to form positive associations upon pairing food with the chemoattractant butanone. Increasing the number of conditioning periods induces long-...

Mated Reproductive Span

Mated Reproductive Span

For measuring the length of the reproductive span in mated C. elegans

Reproductive Span Chip

Reproductive Span Chip

A microfluidic device and automatic counting system for the study of C. elegans reproductive aging.

Tissue-specific Sequencing

Tissue-specific Sequencing

For the separation, sorting, and sequencing of GFP labeled tissues in C. elegans for transcriptional profiling.

CeAid (C. elegans Application for inputting data)

CeSnAP (C. elegans Snapshot Analysis Platform)

Learning & Memory

Mated Reproductive Span

Reproductive Span Chip

Tissue-specific Sequencing