Assays performed on model organisms, including C. elegans, often require a large sample size and daily recording of data. To minimize the amount of time that the experimenter spends logging data, we developed CeAid (C. elegans Application for inputting data) to automate the data entry process [1]. Implementing…
We have created a ML-based algorithm, CeSnAP, paired with a rapid snapshot acquisition technique that serves as a simple and versatile workflow to quantify C. elegans behavioral phenotypes [1]. CeSnAP can be used for smart analysis of videos or snapshots upon training of a convolutional neural network (C-NN). The…
Here we describe methods to test C. elegans associative learning and short- and long-term associative memory. These population assays employ the worm's ability to chemotax toward volatile odorants, and to form positive associations upon pairing food with the chemoattractant butanone. Increasing the number of…
For measuring the length of the reproductive span in mated C. elegans
Generate a population of Wild Type C. elegans hermaphrodites and males via heat shock and propagation. Sync a population of the strain to be tested (usually done via bleaching, although one can pick from a mixed population if necessary.) When utilizing the…
A microfluidic device and automatic counting system for the study of C. elegans reproductive aging.
A Microfluidic Chip for Reproductive Span Measurement in C. elegans Method detailed in:Li, S., Stone, H.A., and Murphy, C.T. A microfluidic device and automatic counting system for the study of C. elegans…
For the separation, sorting, and sequencing of GFP labeled tissues in C. elegans for transcriptional profiling.
Method detailed in:Kaletsky, R.†, Lakhina, V.†, Arey, R., Williams, A., Landis, J., Ashraf, J., and Murphy, C.T. The C. elegans adult neuronal IIS/FOXO transcriptome reveals adult phenotype regulators.