Cell-Type-Specific Transcriptome Analysis in the Drosophila Mushroom Body Reveals Memory-Related Changes in Gene Expression.

TitleCell-Type-Specific Transcriptome Analysis in the Drosophila Mushroom Body Reveals Memory-Related Changes in Gene Expression.
Publication TypeJournal Article
Year of Publication2016
AuthorsCrocker A, Guan X-J, Murphy CT, Murthy M
JournalCell Rep
Volume15
Issue7
Pagination1580-1596
Date Published2016 05 17
ISSN2211-1247
KeywordsAnimals, Drosophila melanogaster, Gene Expression Profiling, Gene Expression Regulation, Gene Ontology, Genes, Insect, Memory, Mushroom Bodies, Neurons, Receptors, Cell Surface, Reproducibility of Results, RNA, Messenger, Sequence Analysis, RNA
Abstract

Learning and memory formation in Drosophila rely on a network of neurons in the mushroom bodies (MBs). Whereas numerous studies have delineated roles for individual cell types within this network in aspects of learning or memory, whether or not these cells can also be distinguished by the genes they express remains unresolved. In addition, the changes in gene expression that accompany long-term memory formation within the MBs have not yet been studied by neuron type. Here, we address both issues by performing RNA sequencing on single cell types (harvested via patch pipets) within the MB. We discover that the expression of genes that encode cell surface receptors is sufficient to identify cell types and that a subset of these genes, required for sensory transduction in peripheral sensory neurons, is not only expressed within individual neurons of the MB in the central brain, but is also critical for memory formation.

DOI10.1016/j.celrep.2016.04.046
Alternate JournalCell Rep
PubMed ID27160913
PubMed Central IDPMC5047377
Grant ListDP2 NS092378 / NS / NINDS NIH HHS / United States
T32 MH065214 / MH / NIMH NIH HHS / United States