Comparing genomic expression patterns across species identifies shared transcriptional profile in aging.

TitleComparing genomic expression patterns across species identifies shared transcriptional profile in aging.
Publication TypeJournal Article
Year of Publication2004
AuthorsMcCarroll SA, Murphy CT, Zou S, Pletcher SD, Chin C-S, Jan YNung, Kenyon C, Bargmann CI, Li H
JournalNat Genet
Volume36
Issue2
Pagination197-204
Date Published2004 Feb
ISSN1061-4036
KeywordsAge Factors, Aging, Animals, Caenorhabditis elegans, Databases, Genetic, Drosophila melanogaster, Gene Expression Profiling, Gene Expression Regulation, Humans, Oligonucleotide Array Sequence Analysis, Phylogeny, Saccharomyces cerevisiae, Time Factors
Abstract

We developed a method for systematically comparing gene expression patterns across organisms using genome-wide comparative analysis of DNA microarray experiments. We identified analogous gene expression programs comprising shared patterns of regulation across orthologous genes. Biological features of these patterns could be identified as highly conserved subpatterns that correspond to Gene Ontology categories. Here, we demonstrate these methods by analyzing a specific biological process, aging, and show that similar analysis can be applied to a range of biological processes. We found that two highly diverged animals, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, implement a shared adult-onset expression program of genes involved in mitochondrial metabolism, DNA repair, catabolism, peptidolysis and cellular transport. Most of these changes were implemented early in adulthood. Using this approach to search databases of gene expression data, we found conserved transcriptional signatures in larval development, embryogenesis, gametogenesis and mRNA degradation.

DOI10.1038/ng1291
Alternate JournalNat. Genet.
Full Text
PubMed ID14730301