Title | Transcriptome analysis of adult Caenorhabditis elegans cells reveals tissue-specific gene and isoform expression. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Kaletsky R, Yao V, Williams A, Runnels AM, Tadych A, Zhou S, Troyanskaya OG, Murphy CT |
Journal | PLoS Genet |
Volume | 14 |
Issue | 8 |
Pagination | e1007559 |
Date Published | 2018 Aug |
ISSN | 1553-7404 |
Abstract | The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans' four major tissues (or "tissue-ome"), identifying ubiquitously expressed and tissue-specific "enriched" genes. These data newly reveal the hypodermis' metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms. Finally, we developed a machine learning-based prediction tool for 76 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-β activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals. |
DOI | 10.1371/journal.pgen.1007559 |
Alternate Journal | PLoS Genet. |
Full Text | |
PubMed ID | 30096138 |
PubMed Central ID | PMC6105014 |